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Key Documents

50734-U

Supelco

Ascentis® Express 90 Å RP-Amide (5 μm) HPLC Columns

L × I.D. 5 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Pricing and availability is not currently available.

Product Name

Ascentis® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

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1 of 4

This Item
SAB4200714H4149WH0003304M2
Gene Information

human ... HSPA1A(3303), HSPA1B(3304)
rat ... Hspa1a(24472), Hspa1b(294254)

Gene Information

human ... HSPA1A(3303)
mouse ... HSPA1A(193740)
rat ... HSPA1A(24472)

Gene Information

human ... HSPD1(3329)
rat ... Hspd1(63868)

Gene Information

human ... HSPA1B(3304)

biological source

mouse

biological source

-

biological source

mouse

biological source

mouse

clone

BRM-22, monoclonal

clone

BRM-22, monoclonal

clone

LK1, monoclonal

clone

3B7, monoclonal

species reactivity

bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster

species reactivity

plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse

species reactivity

rat, human, chicken

species reactivity

mouse, human, rat

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

General description

Ascentis Express 5 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.6 μm thick porous shell and the highly uniform overall particle size of 5 μm. The one-step bonded, endcapped, amide-based, polar-embedded stationary phase of Ascentis Express 5 μm RP-Amide provides a stable, reversed-phase packing with decreased hydrophobic character. Ascentis Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from “phase collapse”.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Martin Mzik et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1020, 85-89 (2016-04-01)
A simple, rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the quantitative determination in rat plasma of a new candidate for AD treatment, namely PC 48 (a 7-MEOTA-donepezil like compound) in
Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
Hirotaka Kushida et al.
Biomedical chromatography : BMC, 27(12), 1647-1656 (2013-07-03)
Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high-performance liquid

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