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50487-U

Supelco

Ascentis® Express 90 Å Phenyl-Hexyl (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core Phenyl HPLC column

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1 UNIT
CN¥14,517.30

CN¥14,517.30

Estimated to ship onJune 03, 2025Details

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1 UNIT
CN¥14,517.30

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

CN¥14,517.30

Estimated to ship onJune 03, 2025Details

Product Name

Ascentis® Express Phenyl-Hexyl, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L11

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

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This Item
C3330180038D23408
6-甲基-1,3,5-三嗪-2,4-二胺 98%

407801

6-甲基-1,3,5-三嗪-2,4-二胺

2-氯-4,6-二氨-1,3,5-三嗪 95%

C33301

2-氯-4,6-二氨-1,3,5-三嗪

三聚氰胺-15N3) analytical standard

80038

三聚氰胺-15N3)

2,4-二氨基-6-苯基-1,3,5-三嗪 97%

D23408

2,4-二氨基-6-苯基-1,3,5-三嗪

assay

98%

assay

95%

assay

≥95.0% (GC)

assay

97%

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

mp

274-276 °C (lit.)

mp

>320 °C (lit.)

mp

>300 °C (lit.)

mp

219-221 °C (lit.)

form

powder

form

powder

form

-

form

powder

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Application


  • A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This study by Lhotská et al. explores the application of a fused-core column combined with on-line SPE-HPLC for rapid and automated analysis of ochratoxin A and citrinin in lager beers. The Ascentis® Express Phenyl-Hexyl column demonstrates high efficiency and selectivity, significantly improving the throughput and sensitivity of the analysis (Lhotská et al., 2016).

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    USXO001424
    USXO001423
    USXO001422
    USXO001421
    USXO001420

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. Can a 100% aqueous mobile phase be used with the Phenyl-Hexyl HPLC column?

      The phenyl-hexyl phase is 100% aqueous compatible.

    6. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    C M Chavez-Eng et al.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
    An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
    E Lesellier
    Journal of chromatography. A, 1266, 34-42 (2012-11-03)
    The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
    Petr Chocholouš et al.
    Talanta, 103, 221-227 (2012-12-04)
    Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
    Ivona Lhotská et al.
    Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
    A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
    Alex D Batista et al.
    Talanta, 133, 142-149 (2014-12-02)
    On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
    View All Related Papers

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