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Ascentis® Express 90 Å HILIC (5 μm) HPLC Columns

L × I.D. 5 mm × 4.6 mm, HPLC Guard Cartridge, pkg of 3 ea

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UNSPSC Code:
41115700
NACRES:
SB.52

product name

Ascentis® Express HILIC, 5 μm Guard Cartridge, 5 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

material

stainless steel column

Quality Level

100

Agency

suitable for USP L3

product line

Ascentis®

feature

endcapped: no

packaging

pkg of 3 ea

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

5 mm × 4.6 mm

surface area

90 m2/g

matrix

superficially porous particle

matrix active group

silica phase

particle size

5 μm

pore size

90 Å

operating pH

1-8

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

Ascentis® Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis Express columns. These Ascentis Express guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridges are packed with Ascentis Express Fused-Core® particles. Order guard column holder separately.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Product No.
Description
Pricing

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What steps should be taken to maximize column life?

    To maximize column life, ensure that samples and mobile phases are particle-free. The use of guard columns or an in-line filter with 0.5 μm porosity between the sample injector and the column is highly recommended. The 2 μm porosity frits on Ascentis Express 5 μm HILIC columns are less subject to pluggage than are the 0.5 μm frits typically used with other small-particle columns. Should the operating pressure of the column suddenly increase beyond normal levels, reversing the flow direction of the column may be attempted to remove debris on the inlet frit.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Paweł Kubica et al.
Journal of pharmaceutical and biomedical analysis, 127, 184-192 (2016-01-20)
Hydrophilic interaction liquid chromatography (HILIC) coupled with tandem mass spectrometry (MS/MS) was used to separate artificial and natural sweeteners approved for use in European Union (EU). Among three tested HILIC columns (BlueOrchid PAL-HILIC, Ascentis Express Si and Acclaim™ Trinity™ P2)
Imran Ali et al.
Biomedical chromatography : BMC, 26(8), 1001-1008 (2012-01-13)
Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available
Tiziana Bertolini et al.
Journal of chromatography. A, 1365, 131-139 (2014-09-23)
A simple, sensitive and fast hydrophilic interaction liquid chromatography (HILIC) method using ultraviolet diode-array detector (UV-DAD)/electrospray ionization tandem mass spectrometry was developed for the automated high performance liquid chromatography (HPLC) determination of sodium risedronate (SR) and its degradation products in
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl
Alexandre Grand-Guillaume Perrenoud et al.
Journal of chromatography. A, 1360, 275-287 (2014-08-19)
Superficially porous particles (SPP), or core shell particles, which consist of a non-porous silica core surrounded by a thin shell of porous silica, have gained popularity as a solid support for chromatography over the last decade. In the present study

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