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LiChrospher® RP-18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Synonym(s):

TM=["LiChrospher"] C18 HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

LiChrospher® RP-18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L1

product line

LiChrospher®

feature

endcapped: no

manufacturer/tradename

LiChrospher®

packaging

1 ea of

extent of labeling

21% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4 mm

surface area

350 m2/g

matrix

octadecyl
silica particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å

operating pH

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

LiChrospher® RP-18 HPLC column (L × I.D. 25 cm × 4 mm) is found to be reliable and high versatile. Suitable for reversed-phase high performance liquid chromatography (RP-HPLC) of acidic, neutral and weakly basic compounds, according to L1 USP specifications.

Application


  • HPLC-UV and GC-MS Methods for Determination of Chlorambucil and Valproic Acid in Plasma for Further Exploring a New Combined Therapy of Chronic Lymphocytic Leukemia.: This study developed and validated HPLC-UV and GC-MS methods for determining chlorambucil and valproic acid in plasma, aiming to explore their combined therapy for chronic lymphocytic leukemia. The LiChrospher® RP-18 HPLC Column was instrumental in achieving the separation and quantification (Lipska et al., 2021).

  • Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma.: This research utilized the LiChrospher® RP-18 HPLC Column for the rapid determination of metronidazole and its metabolite in murine blood plasma, highlighting its effectiveness in pharmacokinetic studies (Zemanová et al., 2022).

  • Application of chemometric approach for development and validation of high performance liquid chromatography method for estimation of ropinirole hydrochloride.: This paper presents a chemometric approach to developing and validating an HPLC method for estimating ropinirole hydrochloride, utilizing the LiChrospher® RP-18 column for precise and accurate results (Fatima et al., 2019).

  • A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography.: The study developed a novel solid-phase extraction method for determining pantoprazole in human plasma using HPLC with the LiChrospher® RP-18 column, achieving high sensitivity and specificity (Zendelovska et al., 2019).

  • Application of an HPLC-MS/MS method for Teicoplanin drug substance and related impurities, part 2: Identity assignment of related impurities.: This research employed the LiChrospher® RP-18 column in an HPLC-MS/MS method to identify and assign related impurities in teicoplanin drug substances, demonstrating the column′s capability in impurity profiling (Tengattini et al., 2019).

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Legal Information

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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