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33027

Supelco

BSTFA, Derivatization Grade

for GC derivatization

Synonym(s):

N,O-Bis(trimethylsilyl)trifluoroacetamide

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About This Item

CAS Number:
EC Number:
UNSPSC Code:
41116105

grade

for GC derivatization

reaction suitability

reagent type: derivatization reagent
reaction type: Acylations

reagent type: derivatization reagent
reaction type: Silylations

packaging

pkg of 25 mL

technique(s)

GC/MS: suitable

InChI

1S/C8H18F3NOSi2/c1-14(2,3)12-7(8(9,10)11)13-15(4,5)6/h1-6H3

InChI key

XCOBLONWWXQEBS-UHFFFAOYSA-N

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General description

BSTFA is a derivatizing agent widely used in the derivatization of estrogenic steroids, thereby resulting in the formation of trimethylsilyl (TMS) derivative. It is also known to react with the aromatic hydroxyl group.

Application

BSTFA was used for silylation of samples during GC-MS analysis of Glutathione conjugates of Prostaglandin A1.
Suitable for the derivatization of Υ-aminobutyric acid, n-acetylneuraminic acid, arginine, dipeptides, glycine, glycosphingolipi, n-hydroxylamines, lysine, nitrilotriacetate, phenols (sterically hindered), phospholipids, phosphoserine and phosphothreonine, prostaglandins F, prostaglandin H2 methyl ester, prostaglandins F2α, sulfur amino acids, terephthalate prepolymer mixture, thiohydantoins of amino acids, and thromboxane B2.

Other Notes

Reagent for N-acetyl, O-trimethylesilyl, trimethylsilyl, trimethylsilyl diglyceride, trimethylsilyl ether, sulfonyl trimethylsilyl esters and trimethylsilyl thiohydantoins.

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Pictograms

FlameExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

93.2 °F

Flash Point(C)

34 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

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L M Cagen et al.
The Journal of biological chemistry, 251(21), 6550-6554 (1976-11-10)
When prostaglandin A1 was incubated with a Tris/saline suspension of washed human red blood cells, a substantial amount was converted to polar metabolites. These were purified by solvent extraction, XAD-2 column, and cellulose thin layer chromatography and characterized by chromatography
N.E. Hoffman and K.A. Peteranetz
Analytical Letters, 5, 589a-589a (1972)
C.W. Gehrke and K. Leimer
Journal of Chromatography A, 57, 219-219 (1971)
E.R.Atkinson and S.I. Calouche
Analytical Chemistry, 43, 460-460 (1971)
Rapid determination of twenty amino acids by gas chromatography.
J P Hardy et al.
Analytical chemistry, 44(8), 1497-1499 (1972-07-01)

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