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23348-U

Supelco

SP®-2560 Capillary GC Column

L × I.D. 75 m × 0.18 mm, df 0.14 μm

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Synonym(s):
GC column, biscyanopropyl siloxane
UNSPSC Code:
12352211
eCl@ss:
32119290
NACRES:
SB.54

material

fused silica

Quality Level

100

Agency

AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5

parameter

≤25-250 °C temperature (isothermal or programmed)

Beta value

321

df

0.14 μm

technique(s)

gas chromatography (GC): suitable (fast GC)

L × I.D.

75 m × 0.18 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

application(s)

agriculture
clinical
food and beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Application

SP®-2560 capillary GC column may be used for analysing fatty acid methyl esters (FAME) using capillary gas liquid chromatography (GLC).

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

Regulatory Information

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S Pegolo et al.
Journal of dairy science, 100(4), 2564-2576 (2017-02-13)
Buffalo milk is the world's second most widely produced milk, and increasing attention is being paid to its composition, particularly the fatty acid profile. The objectives of the present study were (1) to characterize the fatty acid composition of Mediterranean
Determination ofcis andtrans-Octadecenoic acids in margarines by gas liquid chromatography-infrared spectrophotometry.
Ratnayake, W. M. N., et al.
Journal of the American Oil Chemists' Society, 67, 804-810 (1990)
W M Ratnayake
Journal of AOAC International, 78(3), 783-802 (1995-05-01)
An infrared spectrophotometric (IR) method for the determination of total trans unsaturated fatty acid (trans) content and a combined gas-liquid chromatographic/infrared spectrophotometric (GC/IR) method for determination of fatty acid composition of partially hydrogenated vegetable oils (PHVO) were studied collaboratively in
Carla Ragonese et al.
Analytical chemistry, 81(13), 5561-5568 (2009-06-02)
The present research is focused on the evaluation of one 0.10 mm i.d. and two 0.25 mm i.d., ionic liquid (IL) stationary phase [1,9-di(3-vinyl-imidazolium) nonane bis(trifluoromethyl) sulfonyl imidate] columns, with lengths of 12 (the microbore capillary), 30 and 100 m
R R Gangidi et al.
Lipids, 39(6), 577-582 (2004-11-24)
Conjugated linoleic acid (CLA), an anticarcinogenic compound with numerous other health benefits, is present mainly in dairy and beef lipids. The main CLA isomer present in dairy and beef lipids is cis 9, trans 11 CLA at a 0.5% concentration.
View All Related Papers

Articles

New cis/trans FAME Standard for GC Optimization

AOCS and AOAC methods for trans fatty acid composition require highly polar cyanosilicone capillary columns.

Protocols

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Chromatograms

application for SPE, suitable for GC

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