21278-U
GC Stationary Phase
phase OV-275, bottle of 5 g
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packaging
bottle of 5 g
parameter
25-250 °C temp. range
technique(s)
gas chromatography (GC): suitable
matrix active group
OV-275 phase
solubility
acetone: soluble
column type
packed GC
General description
GC methods are divided into two classes depending on the nature of stationary phases; gas-solid chromatography (GSC) and gas-liquid chromatography (GLC). GSC has solid adsorptive material and solute particles are removed from mobile phase by electrostatic forces. GLC has a thin layer of liquid coated or bonded on the surface of an inert particle or on the walls of the column where solute particles are retained in the liquid phase based on their partition coefficients. The primary necessity of a stationary phase is to provide sample separation sustaining phase integrity over a reasonable period of time. It should be stable for the chemical and thermal changes. Selectivity, peak symmetry, analysis time, degree of separation, peak tailing are a few parameters that should be considered in order to choose a stationary phase. OV-275 is a highly polar silicone.
Synthesized specifically to be purer, of narrow molecular weight range, and without trace catalysts or impurities for use as a GC stationary phase.
Application
OV-275 on Chromosorb WAW packed glass column is suitable for analysis of fatty acid composition of total lipid and major lipid classes, using gas-liquid chromatography.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Chromatography and Separation Science, 139-139 (2003)
Clinical chemistry, 42(3), 454-461 (1996-03-01)
Blood cell and plasma lipid classes and their fatty acids were analyzed in a child with X-linked adrenoleukodystrophy. The increase in saturated fatty acids with very long chains typical of this disease occurred almost exclusively in sphingomyelin. In this lipid
Remington: The Science and Practice of Pharmacy, 605-605 (2006)
Methods of Air Sampling and Analysis, 98-98 (1988)
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