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Supelco

GC Stationary Phase

phase OV-225, bottle of 5 g

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UNSPSC Code:
23151817

Agency

meets requirements for USP G19

packaging

bottle of 5 g

parameter

0-265 °C temp. range

technique(s)

gas chromatography (GC): suitable

matrix active group

OV-225 phase

solubility

acetone: soluble

column type

packed GC

General description

GC methods are divided into two classes depending on the nature of stationary phases; gas-solid chromatography (GSC) and gas-liquid chromatography (GLC). GSC has solid adsorptive material and solute particles are removed from mobile phase by electrostatic forces. GLC has a thin layer of liquid coated or bonded on the surface of an inert particle or on the walls of the column where solute particles are retained in the liquid phase based on their partition coefficients. The primary necessity of a stationary phase is to provide sample separation sustaining phase integrity over a reasonable period of time. It should be stable for the chemical and thermal changes. Selectivity, peak symmetry, analysis time, degree of separation, peak tailing are a few parameters that should be considered in order to choose a stationary phase. OV-225 is a polar siliconised phase. It has 50% methysilicone, 25% cyanopropyl and 25% phenyl-silicone. It is possible to separate aromatic compounds from aliphatic ones containing the same number of carbons.
Synthesized specifically to be purer, of narrow molecular weight range, and without trace catalysts or impurities for use as a GC stationary phase.

Application

OV-225 on columns is suitable for resolving neutral and amino sugars during chemical analysis using gas chromatograph.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S J Millar et al.
Infection and immunity, 51(1), 302-306 (1986-01-01)
Two separate species of lipopolysaccharide (LPS) from Bacteroides gingivalis 381 have been isolated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated not only the heterogeneity of each species, but also that they represented high- and low-molecular-weight LPS entities. Although both contained the
Michael O'Keefe
Residue Analysis in Food: Principles and Applications, 187-187 (2000)
James P. Lodge, Jr.
Methods of Air Sampling and Analysis, 98-98 (1988)
David B. Troy, Paul Beringer
Remington: The Science and Practice of Pharmacy, 605-605 (2006)

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