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Supelco

Supelite™ DAX-8

jar of 100 g

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Synonym(s):
XAD-8
UNSPSC Code:
23151817

form

solid

packaging

jar of 100 g

technique(s)

HPLC: suitable
LPLC: suitable
ion chromatography: suitable

surface area

140 m2/g

matrix

acrylic ester

matrix active group

polymer

particle size

40-60 mesh

pore size

~0.79 mL/g pore volume
225 Å mean pore size

density

1.09 g/mL at 25 °C (true wet)(lit.)
1.23 g/mL at 25 °C (skeletal)(lit.)

separation technique

reversed phase

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General description

Supelite™ DAX-8 is a nonfunctionalised resin with strong hydrophobic organic matter endorsed to humic and fulvic acids. It is also referred as polymethylmethacrylate resin.

Application

Supelite™ DAX-8 was used as an absorbent in:
  • HPLC-SEC fractionation system
  • column chromatography
  • extraction procedure and extracts were analysed using Cary Eclipse fluorescence spectrophotometer
Adsorbent resin with moderate polarity for compounds up to 150,000 MW. Most commonly used for adsorption of fulvic and humic acids, treatment of paper pulp mill waste, and removal of alcohols, surfactants, and colorants from samples.

Preparation Note

DAX-8 is a salt imbibed resin, which requires some preparation prior to use. In the protocol below, you may use methanol in place of ethanol.
  • First, rinse the adsorbent resin with 2-3 bed volumes (BV) of distilled water.
  • Soak overnight in 2 BV of 95% ethanol.
  • After soaking, the resin is introduced into the column and rinsed with an additional 2 BV of 95% ethanol.
  • Rinse with 2 BV of distilled water to displace the ethanol, followed by 1 BV of 4% (w/v) sodium hydroxide, 2 BV of distilled water, 1 BV 4% (v/v) hydrochloric acid, and finally by distilled water until the pH of the eluent becomes neutral.

Legal Information

Supelite is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

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Lucas B Harrington et al.
Cell, 170(6), 1224-1233 (2017-08-29)
CRISPR-Cas9 proteins function within bacterial immune systems to target and destroy invasive DNA and have been harnessed as a robust technology for genome editing. Small bacteriophage-encoded anti-CRISPR proteins (Acrs) can inactivate Cas9, providing an efficient off switch for Cas9-based applications.
Sorption of aquatic humic matter by DAX-8 and XAD-8 resins: comparative study using pyrolysis gas chromatography
Peuravuori, Juhani, Tero Lehtonen, and Kalevi Pihlaja.
Analytica Chimica Acta, 471, 219-226 (2002)
Hua Zhang et al.
The Science of the total environment, 407(2), 879-886 (2008-11-15)
Dissolved organic matter (DOM) in leachate samples collected at Laogang Sanitary Landfill in Shanghai, China were fractionated using size exclusion chromatography and were characterized using estrogen receptor-alpha competitor screening assay as toxicity activity index. The estrogen activity of leachate was
The fouling of microfiltration membranes by NOM after coagulation treatment
Carroll, T., et al.
Water Research, 34, 2861-2868 (2000)

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