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form
solid
packaging
bucket of 500 g
technique(s)
LPLC: suitable
UV/Vis spectroscopy: suitable
surface area
~500 m2/g
matrix
styrene-divinylbenzene
matrix active group
polymer
particle size
50-100 μm
pore size
~1.01 mL/g pore volume
260 Å mean pore size
density
1.30 g/mL at 25 °C (true wet)(lit.)
separation technique
reversed phase
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General description
Sepabeads® SP-20SS is an epoxy-activated hydrophobic styrene-divinylbenzene polymer resin.
Application
- Sepabeads® SP-20SS was used as adsorbent for bovine serum albumin on allyl glycidyl ether-ethylene glycol dimethacrylate copolymer using UV-visible spectrophotometry.
- Sepabeads® SP-20SS was used as column packing material for purification of peptides.
- Sepabeads® SP-20SS was used to remove active laccase from Heterobasidion annosum.
Polyaromatic adsorbent resin for separation of hydrophobic compounds: small and medium proteins, peptides, amino acids. Excellent for reversed phase applications.
Other Notes
Swelling in toluene = 30%
Legal Information
Sepabeads is a registered trademark of Mitsubishi Chemical Corp.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Jeong-Nam Park et al.
Applied and environmental microbiology, 77(4), 1187-1195 (2010-12-25)
Mannosylphosphorylation of N- and O-glycans, which confers negative charges on the surfaces of cells, requires the functions of both MNN4 and MNN6 in Saccharomyces cerevisiae. To identify genes relevant to mannosylphosphorylation in the dimorphic yeast Yarrowia lipolytica, the molecular functions
J Ihssen et al.
Journal of applied microbiology, 110(4), 924-934 (2011-01-18)
Wild-type white rot fungi are the most important production organisms for laccase, a promising oxidative biocatalyst with numerous applications. This study aimed at identifying novel highly productive strains, finding optimal cultivation conditions for laccase production and establishing a simple immobilization
Lakshmi Swarnalatha Jasti et al.
Biotechnology progress, 30(2), 317-323 (2014-01-23)
Allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) shows excellent bovine serum albumin (BSA) adsorption (up to 16% (w/w)) at pH 8.0 and the adsorbed BSA is strongly bound. This protein-coated polymer provides a novel
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