Anti-p-ErbB4 (Tyr1284) Antibody, clone 4C17 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Receptor tyrosine-protein kinase erbB-4 (UniProt: Q15303; also known as EC:2.7.10.1, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4) is encoded by the ERBB4 (also known as HER4) gene (Gene ID: 2066) in human. ErbB4 is a tyrosine-protein kinase that serves as a receptor for neuregulins and EGF family members. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 26-651), a transmembrane domain (aa 652-675), and a cytoplasmic domain (aa 676-1308). Its protein kinase domain is localized in amino acids 718-985. ErbB4 can be proteolytically processed to generate an intracellular domain (4ICD) that can translocate to nucleus to promote import and activation of STAT5A. 4ICD also translocates to mitochondria where it promotes apoptosis. ErbB4 is highly expressed in brain, heart, kidney, skeletal muscle, spleen, testis, and breast tissue. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. It is also required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Ligand binding to ErbB4 triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. It is constitutively phosphorylated at a basal level and ligand binding LEADS to increased phosphorylation. Phosphorylation at Tyr-1035 is important for its interaction with STAT1 and phosphorylation at Tyr-1056 is required for its interaction with PIK3R1. Its phosphorylation at Tyr-1242 is important for interaction with SHC adaptor protein 1 (SHC1). Phosphorylation of ErbB4 at Tyrosine 1284 plays a crucial role in modulating its signaling pathways, particularly in response to neuregulin ligands. This modification is essential for the activation of downstream signaling cascades, including the PI3K/Akt and MAPK pathways, which are pivotal for cellular processes such as proliferation, differentiation, and survival. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, Affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Williams, CC., et al. (2004). J. Cell Biol. 167(3); 469-478; Komuro, A., et al. (2003). J. Biol. Chem. 278(35); 33334-33341; Harari, D., et al. (1999). Oncogene. 18(17); 2681-2689).

KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphotyrosine 1284 from the C-terminal cytoplasmic domain of human ErbB4.

Quality Control Testing

Evaluated by Western Blotting in lysate from A-431 cells treated with EGF.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ErbB4 phosphorylated at (Tyr1284) in lysate from A-431 cells treated with EGF (50 ng/ml for 10 min) but, not in lysate from untreated A-431 cells.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ErbB4 phosphorylated at (Tyr1284) in serum starved A431 cells treated with EGF (50ng/ml EGF for 10 min).

Peptide Inhibition Assay: Target band detection in lysate from A-431 cells treated with EGF (50 ng/ml for 10 min) was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide but not the corresponding non-phosphopeptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 4C17 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects ErbB4 phosphorylated at Tyrosine 1284.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.