Anti-POLQ Antibody, clone 2F5 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 2F5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects DNA polymerase theta (POLQ). It targets an epitope within 23 amino acids from the N-terminal half.

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human DNA polymerase theta (POLQ).

Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected POLQ in U2OS cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected POLQ in Raji cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected POLQ in Human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected POLQ in U2OS cells.

Affinity Binding Assay: A representative lot of this antibody bound POLQ peptide with a KD of 1.8 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

DNA polymerase theta (UniProt: O75417; also known as EC:2.7.7.7, DNA polymerase eta, POLQ) is encoded by the POLQ (also known as POLH) gene (Gene ID: 10721) in human. POLQ is a homomultimeric enzyme that is minimally expressed in normal tissues but is overexpressed in tumors. Its expression is observed in testis tissue. It contains a helicase-like domain at its N-terminus and a polymerase domain at its C-terminus. They are separated by a large unstructured central domain. It promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery that is triggered in response to double-strand breaks in DNA. It acts by binding directly the 2 ends of resected double-strand breaks, allowing micro homologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. It requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. POL

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.