Anti-Trk-B Antibody, clone 2B22 ZooMAb^® Rabbit Monoclonal

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ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

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Clone 2B22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Trk-B. It targets an epitope within 18 amino acids from the extracellular domain.

His-tagged Recombinant Protein corresponding to the extracellular domain of Human Trk-B (BDNF receptor).

Anti-Trk-B, clone 2B22 ZooMAb, Cat. No. ZRB1281, is a recombinant Rabbit monoclonal antibody that specifically targets Trk-B and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Trk-B in BDNF recombinant protein, mouse brain and rat brain lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected human recombinant Trk-B in human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound human recombinant Trk-B fragment with a KD of 6.8 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Trk-B in E18 cortical cells and PC12 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

BDNF/NT-3 growth factors receptor (UniProt: Q16620; also known as EC:2.7.10.1, GP145-TrkB, Trk-B, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B) is encoded by the NTRK2 (also known as TRKB) gene (Gene ID: 4915) in human. TrkB is a single-pass type I membrane protein that is internalized to endosomes upon ligand-binding. It is synthesized with a signal peptide (aa 1-31) that is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 32-430), a transmembrane domain (aa 431-454), and a cytoplasmic domain (aa 455-822). TrkB is a receptor tyrosine kinase that is involved in the development and maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. In the central nervous system, its expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibula, and multiple facial structures. Upon ligand-binding, it undergoes homodimerization, autophosphorylation, and activation. It can then recruit, phosphorylate and/or activate several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. TrkB is also reported to play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. Following binding of BDNF to TrkB receptor, Tyrosine 816 is phosphorylated, which allows the binding of phospholipase C gamma and activation of signaling cascade. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.