Millipore^® Ziptips

Millipore ZipTips: Sample Preparation of Peptides or Proteins Prior to Electrospray/Nanoelectrospray MS

ZipTip Advantages:

• Single-step desalting, concentration, and purification
• Fractionate complex samples for more meaningful data
• Ideal for peptides, proteins, nucleic acids, and more
• No dead volume for maximum recovery
• Eliminates time-consuming chromatography

Examples of analyses enhanced by ZipTip sample preparation:

• Mass spectrometry
• HPLC (high performance liquid chromatography)
• Capillary electrophoresis
• Atomic absorption spectroscopy

The ZipTip is a 10 μL (P-10) pipette tip with a bed of chromatography media fixed at its end such that there is no dead volume. It is intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality. The sample is aspirated and dispensed through ZipTip to bind, wash, and elute. Recovered samples are contaminant-free and eluted in 0.5-4 μL for direct transfer to a mass spectrometer target or vial.

ZipTip C₁₈ is a 10 μL pipette tip with approximately a 0.6 μL bed of chromatography media fixed at its end such that there is no dead volume. ZipTip Micro-C₁₈ is a 10 μL pipette tip with approximately a 0.2 μL bed of chromatography media fixed at its end such that there is no dead volume. Both are ideal for concentrating and purifying peptides, proteins or oligonucleotides in seconds prior to mass spectrometry, HPLC, capillary electrophoresis and other analytical techniques.

Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany