Protein Microarray Technology

This book is the first of its kind in the field of protein microarrays and addresses novel strategies for constructing highly functional and biocompatible microarrays for screening proteins. The authors provide a roadmap for solving the complex challenges that are currently faced while monitoring protein-protein interactions over a wide range of microarray platforms. In doing so, they also offer a comprehensive overview of microarray surface chemistry, detection technologies, fabrication options for array development, and data analysis of numerous types of protein interactions. Topics covered include: Types of biomolecular interactions; Surface chemistry; Detection technologies; Spotting technologies; Bioinformatics/data analysis.

1. Protein Microarrays: From Fundamental Screening to Clinical Diagnostics
1.1 Potential Need for Protein Microarrays.
1.2 Current Applications of Protein Microarrays.
1.3 Problems and Challenges.
1.4 Potential Solutions: Enabling Technologies and Advancements.
References.
2. Protein Microarray Surface Chemistry and Coupling Schemes
2.1 Introduction.
2.2 Microarray Based of Class Substrates.
2.3 Microarrays based of Gold Substrates.
2.4 Microarrays based on Polymer Substrates.
2.5 Special Formats: Microfluidic Devices and Integrated Semiconductor Chips.
2.6 Chemical Immobilization Techniques for Proteins.
2.7 Conclusions.
References.
3. Optimization of a Protein Microarray Platform Based on a Small-molecule Chemical Affinity System
3.1 Introduction.
3.2 Experimental.
3.3 Results and Discussion. .
3.4 Conclusions.
References.
4. Seein Beneath the Surface of Biomolecular Interactions: Real-time Characterization of Label-free Binding Interactions using Biacore′s Optical Biosensors
4.1 Introduction.
4.2 Biacore Technology.
4.3 Biacore Applications in Basic and Clinical Research.
4.4 Current Developments and Future Perspectives.
References.
5. Surface Plasmon Resonance Imaging Measurements of DNA, RNA, and Protein Interactions to Biomolecular Arrays
5.1 Introduction.
5.2 Surface Plasmon Resonance Imaging.
5.3 Surface Attachment Chemistries.
5.4 SPR Imaging Experiments Using Photopatterned Arrays.
5.5 SPR Imaging Experiments of Arrays Created by Microfluidic Stencils.
5.6 Conclusions.
References.
6. Surface Plasmon Fluorescence Spectroscopy for Protein Binding Studies
6.1 Introduction.
6.2 Fluorescence Profile at the Interface.
6.3 Instrumentation.
6.4 SPR sSignal Conversion.
6.5 Sandwich Detection.
6.6 LOD Evalution.
6.7 Conclusions.
References.
7. The Use of Proteinchip Arrays for Deciphering Biological Pathways
7.1 Introduction.
7.2 Methods to Study Protein Interactions.
7.3 Conclusions and Outlook.
References.
8. Production of Protein Microarrays.
8.1 Introduction.
8.2 From DNA Arrays to Protein Arrays.
8.3 Overview of Protein Microarray Spotting.
8.4 Types of Protein Microarrays.
8.5 Protein Arrayers.
8.6 Surface Chemistry.
8.7 The Arraying Process.
8.8 Detection Issues.
8.9 Validation of Results.
8.10 Stability of Protein Microarrays.
8.11 Future Perspectives and Challenges.
8.12 Conclusion.
References.
9. Nanomechanical Cantilever Sensors for Microarrays (
9.1 Introduction.
9.2 Basic Technology and Instrumentation.
9.3 Cantilever Functionalization.
9.4 Experiments.
9.5 Conclusion and Outlook.
References.
10. Image Analysis Issues and Solution for High-Density Arrays .
10.1 Introduction.
10.2 Image Alignment, Grid Placement, and Spot Location.
10.3 Spatial Segmentation of Signal and Background Pixels.
10.4 Data Quantification.
10.5 Quality Control.
10.6 Batch Automation.
10.7 Experimental Results.
10.8 Conclusion.
References.
Index.