Flow Cytometry Protocols: Methods in Molecular Biology, Vol. 91

This volume includes details on new methods that rely on recently developed instrumentation, without short-changing the time-tested standard techniques. Methods are given for detecting intracellular cytokines, nuclear antigens in leukemia and solid tumors, and cyclins. Protocols are included for performing functional studies like assessing viability, membrane potential, oxidative metabolism, drug resistance, and pharmacokinetics. Nucleic acid methods include cell cycle analysis, chromosome sorting, solid tumor ploidy analysis, apoptosis, and a flow cytometric application of FISH.

Basics of Flow Cytometry.
Detection of Terminal Transferase in Leukemia,
The Use of Flow Cytometry to Detect Intracellular Cytokine Production in Individual Cells.
Detection of Intracellular/Intranuclear Antigens.
Detection of Intracellular/Intranuclear Antigens in Tumor Cells,
Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry.
Estimation of Cell Viability by Flow Cytometry.
Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane Potential.
Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH.
Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry.
Estimation of Drug Resistance by Flow Cytometry,
Flow Cytometric Monitoring of Cellular Pharmacokinetics,
Quantification of Xenoprotein Electroinsertion in Mammalian Cells.
Flow Cytometry Quantification of Electropermeabilization,
Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products.
Cell Cycle Analysis of Asynchronous Populations.
Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies.
Solid Tumor DNA Ploidy Analysis,
FISHing for Cytokines: Methodology Combining Flow Cytometry and In Situ Hybridization.
Analysis of Apoptosis by Flow Cytometry.
Chromosome Sorting and Analysis by FACS.
Simultaneous Five-Six Color Multiparameter Analysis.
Index.