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Y2627

Sigma-Aldrich

2xYT Medium

EZMix® Powder microbial growth medium

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

grade

for molecular biology

description

nutrient rich, quick dissolve, dust free formulation

sterility

non-sterile

form

powder

composition

Inert binder (EZMix only), 0.6 g/L
NaCl, 5 g/L
Tryptone (pancreatic digest of casein), 16 g/L
Yeast extract, 10 g/L

application(s)

agriculture
food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

2xYT EZMix® powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • Easy scale-up using larger package size
  • Standard formulation

Reconstitution

Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

EZMix is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the difference among the 2x YT Media products?

    2x YT Media is available in liquid (Y1003), powder (Y2377) and EZMix™ powder formats (Y2627).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Shuang Yang et al.
eLife, 9 (2020-06-09)
Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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