Skip to Content
Merck
CN
All Photos(1)

Documents

Y2021

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan, and adenine

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
Yeast Synthetic Drop-out Media Supplements
MDL number:
MFCD01095679
UNSPSC Code:
12352200
NACRES:
NA.85

Quality Level

200

form

powder

technique(s)

transformation: suitable

application(s)

microbiology

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.39 g of supplement per liter of medium.

related product

Product No.
Description
Pricing

G7021

D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A5306

Bacteriological agar, for molecular biology

Y1501

Yeast Synthetic Drop-out Medium Supplements, without uracil

Y1376

Yeast Synthetic Drop-out Medium Supplements, without leucine

Y0626

Yeast Nitrogen Base Without Amino Acids, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1876

Yeast Synthetic Drop-out Medium Supplements, without tryptophan

Y1771

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan

Y1896

Yeast Synthetic Drop-out Medium Supplements, without lysine

Y2146

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, and tryptophan

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318

Precautionary Statements

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Corinne A Tovey et al.
Current biology : CB, 28(14), 2314-2323 (2018-07-10)
Microtubules are essential for various cell processes [1] and are nucleated by multi-protein γ-tubulin ring complexes (γ-TuRCs) at various microtubule organizing centers (MTOCs), including centrosomes [2-6]. Recruitment of γ-TuRCs to different MTOCs at different times influences microtubule array formation, but
Elham Alzyoud et al.
Frontiers in cell and developmental biology, 9, 727264-727264 (2021-10-19)
Microtubule nucleation in eukaryotes is primarily promoted by γ-tubulin and the evolutionary conserved protein complex, γ-Tubulin Ring Complex (γ-TuRC). γ-TuRC is part of the centrosome and basal body, which are the best-known microtubule-organizing centers. Centrosomes undergo intensive and dynamic changes

Protocols

Yeast Drop Out

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service