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Sigma-Aldrich

Select Yeast Extract, EZMix® powder

for use in microbial growth medium

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Synonym(s):
Select Yeast Extract
CAS Number:
8013-01-2
MDL number:
MFCD00132599
UNSPSC Code:
12352200
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

InChI

1S/C19H14O2/c20-18(21)11-17-15-8-4-3-7-14(15)16-10-9-12-5-1-2-6-13(12)19(16)17/h1-10,17H,11H2,(H,20,21)

InChI key

GQNBDGXKDJSVGQ-UHFFFAOYSA-N

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General description

Yeast extract is the water-soluble portion of autolyzed yeast, often used in culture media. Yeast extract contains a mixture of amino acids, peptides, water soluble vitamins and carbohydrates.

Application

SelectYeast Extract, EZMix® powder has been used as a component of synthetic municipal wastewater fed to sequencing batch reactor (SBR) for biomass production. It has also been used as a supplement to super broth for the transformation of 5α-competent E. coli cells.
It is also suitable for use as nutritional source in microbial media.
Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

Features and Benefits

Select Yeast Extract EZMix® powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • Easy scale-up using larger package sizes

Other Notes

Water soluble portion of autolyzed yeast with intact B-complex vitamins. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.

Legal Information

EZMix is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jayanta Haldar et al.
Nature protocols, 2(10), 2412-2417 (2007-10-20)
Protocols for the synthesis of the microbicidal polycation N,N-dodecyl,methyl-polyethylenimine and coating (painting) of glass slides with this polycation's butanol solution are described. Subsequently detailed are the procedures for validating that the resultant coated slides are essentially 100% lethal to the
F Morgan-Sagastume et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 69(1), 177-184 (2014-01-18)
Polyhydroxyalkanoates (PHAs) are biodegradable polyesters with comparable properties to some petroleum-based polyolefins. PHA production can be achieved in open, mixed microbial cultures and thereby coupled to wastewater and solid residual treatment. In this context, waste organic matter is utilised as
Artemis Kosta et al.
Methods in molecular biology (Clifton, N.J.), 346, 535-550 (2006-09-08)
In this chapter, we describe how to conveniently demonstrate, assess, and study cell death in Dictyostelium through simple cell culture, clonogenic tests, and photonic (with the help of staining techniques) and electronic microscopy. Cell death can be convniently generated using
Porter M Hall et al.
Nature structural & molecular biology, 29(12), 1217-1227 (2022-12-06)
CRISPR (clustered regularly interspaced short palindromic repeats) utility relies on a stable Cas effector complex binding to its target site. However, a Cas complex bound to DNA may be removed by motor proteins carrying out host processes and the mechanism

Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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