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Y1003

Sigma-Aldrich

2x YT medium

Liquid microbial growth medium

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Synonym(s):
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium
UNSPSC Code:
41106225
NACRES:
NA.85

grade

for molecular biology

Quality Level

300

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.5

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

2xYT liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl

related product

Product No.
Description
Pricing

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the difference among the 2x YT Media products?

    2x YT Media is available in liquid (Y1003), powder (Y2377) and EZMix™ powder formats (Y2627).

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