Product Name
Select Yeast Extract, for use in microbial growth medium
SMILES string
OC(=O)CC1c2c3c(ccc2c4c1cccc4)cccc3
InChI
1S/C19H14O2/c20-18(21)11-17-15-8-4-3-7-14(15)16-10-9-12-5-1-2-6-13(12)19(16)17/h1-10,17H,11H2,(H,20,21)
InChI key
GQNBDGXKDJSVGQ-UHFFFAOYSA-N
grade
Molecular Biology
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
pH
6.5-7.5(10% solution)
solubility
H2O: 100 mg/mL, clear to slightly hazy, yellow to brownish-yellow
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Quality Level
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Related Categories
Application
- as a supplement in the minimal sporulating medium (MSL) for the secretory production of human tumor necrosis factor-α (TNFα) using the carboxypeptidase Y (Cpy) signal peptide.
- in the isolation of halophilic archaea.
- as a component of microbial growth medium for cultivation of marine bacterial strain Zobellia uliginosa.
Suitable for use as nutritional source in microbial media.
General description
Yeast extract is used in all solid media, on which stock cultures are maintained, due to its ability in promoting growth of most bacterial contaminants, thereby making them visible to identify. Yeast extract does not enhance the growth of wild-type cells but essential for auxotrophic mutants.
Other Notes
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Protocols
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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