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Y0396

Sigma-Aldrich

Anti-YB-1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-BP-8, Anti-CSDA2, Anti-CSDB, Anti-DBPB, Anti-MDR-NF1, Anti-NSEP1, Anti-Y BOX binding protein, Anti-YBOX1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells
western blot: 0.5-1 μg/mL using PC12 cell lysates

UniProt accession no.

P67809

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... YBX1(4904)
mouse ... Ybx1(22608)
rat ... Ybx1(500538)

General description

Y-box-binding protein (YB-1) also known as nuclease-sensitive element-binding protein 1 (NSEP1) is a member of the Cold-Sock Domain (CSD) containing proteins. It is composed of three domains: the N-terminal which is thought to function as a trans-activation regulatory domain, a highly conserved nucleic acid binding domain (the CSD), and C-terminal domain, involved in protein-protein interactions.

Specificity

Anti-YB-1 (C-terminal) antibody is specific for YB-1 in rats, mice and humans. The immunizing peptide specifically inhibits the detection of the YB-1 band by immunoblotting.

Immunogen

synthetic peptide corresponding to amino acids 307-324 of human YB-1, conjugated to KLH. The corresponding sequence is identical in rat and mouse YB-1 proteins.

Application

Anti-YB-1 (C-terminal) antibody has been used in
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence
  • double-immunostaining

Biochem/physiol Actions

Y-box-binding protein (YB-1) is shown to bind to the Y-box promoter element to regulate gene expression. It plays an important role in a wide variety of cellular functions such as transcriptional and translation regulation, DNA repair, drug resistance, and stress responses.

Physical form

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts
Wei W J, et al.
Nucleic Acids Research, 40(17), 8622-8636 (2012)
The Y-box binding protein 1 suppresses Alzheimer?s disease progression in two animal models
Bobkova N V, et al.
PLoS ONE, 10(9), e0138867-e0138867 (2015)
Sabine Brandt et al.
Cells, 13(5) (2024-03-13)
Fibrosis is characterized by excessive extracellular matrix formation in solid organs, disrupting tissue architecture and function. The Y-box binding protein-1 (YB-1) regulates fibrosis-related genes (e.g., Col1a1, Mmp2, and Tgfβ1) and contributes significantly to disease progression. This study aims to identify
N V Bobkova et al.
PloS one, 10(9), e0138867-e0138867 (2015-09-24)
The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously
Irina A Eliseeva et al.
International journal of molecular sciences, 25(3) (2024-02-10)
Y-box-binding proteins (YB proteins) are multifunctional DNA- and RNA-binding proteins that play an important role in the regulation of gene expression. The high homology of their cold shock domains and the similarity between their long, unstructured C-terminal domains suggest that
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