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X1875

Xanthine Oxidase from bovine milk

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

Synonym(s):

XOD, Xanthine:oxygen oxidoreductase

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About This Item

CAS Number:
9002-17-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-657-6
MDL number:
MFCD00082145
EC Number:
1.17.3.2.

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Product Name

Xanthine Oxidase from bovine milk, Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

type

Grade I

form

ammonium sulfate suspension

specific activity

≥0.4 units/mg protein

foreign activity

uricase ≤0.5%

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Analysis Note

Protein determined by biuret

Application

Xanthine Oxidase from bovine milk has been used:
  • in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
  • in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
  • as a standard to determine XO activity
  • as a standard to test the synergistic effect of docosahexaenoic acid (DHA)

Biochem/physiol Actions

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.

General description

Formerly E.C. 1.1.3.22
Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.

Other Notes

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
0000512060
0000512061
0000512061
0000512060
0000493960

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Methods of Enzymatic Analysis (2012)
Antioxidant properties of propofol and erythropoietin after closed head injury in rats
Ozturk E, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 29, 922-927 (2005)
Xanthine oxidase: isolation, assays of activity, and inhibition
Kostic DA, et al.
Journal of Chemistry, 2015 (2015)
Xanthine oxidase inhibitors from Archidendron clypearia (Jack.) I.C. Nielsen: Results from systematic screening of Vietnamese medicinal plants
Duong NT, et al.
Asian Pacific Journal of Tropical Medicine, 10(6), 549-556 (2017)
Kurt Warnhoff et al.
Genes & development, 35(3-4), 212-217 (2021-01-16)
The molybdenum cofactor (Moco) is a 520-Da prosthetic group that is synthesized in all domains of life. In animals, four oxidases (among them sulfite oxidase) use Moco as a prosthetic group. Moco is essential in animals; humans with mutations in
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