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X100RS

Sigma-Aldrich

Triton™ X-100 reduced

Synonym(s):

Polyoxyethylene (10) isooctylcyclohexyl ether

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About This Item

Linear Formula:
4-(C8H17)C6H10(OCH2CH2)nOH, n~10
CAS Number:
92046-34-9
MDL number:
MFCD00167048
UNSPSC Code:
12161900
NACRES:
NA.25

biological source

synthetic (oragnic)

Quality Level

100

description

non-ionic

form

viscous liquid

mol wt

micellar average mol wt 80,000
average mol wt 625

aggregation number

100-155

refractive index

n20/D 1.473 (lit.)

CMC

0.2-0.9

mp

48 -50  °C (118 - 122 °F)

transition temp

cloud point 65 °C

solubility

water: soluble, clear to slightly hazy, colorless

density

1.029 g/mL at 25 °C (lit.)

absorption

≤0.25 at 277 nm in H2O at 0.5%

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO

InChI

1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3

InChI key

QQJNBKDKLMCALZ-UHFFFAOYSA-N

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General description

Triton™ X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a vital component in lysis buffers. Widely utilized in biochemical and cell biology research, Triton™ X-100 facilitates the isolation of lipid rafts, enhancing the solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, this product exhibits excellent wetting properties and emulsification enhancement.

In cell biology, Triton™ X-100 plays a crucial role in solubilizing membrane-bound proteins, preserving their native conformation in solution. Furthermore, Triton™ X-100 reduced, resulting from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative, has been associated with potential benefits in enzyme digestion enhancement and effects on the photoisomerization of bacteriorhodopsin. The versatility of Triton™ X-100 in various research applications underscores its significance as a fundamental tool in biochemical and cell biology investigations.

Application

Triton™ X-100 reduced has been used:
  • in nuclear magnetic resonance (NMR) sample tube for lysing/rupturing the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles
  • in cell permeabilization
  • as a component in Tris/HCl to determine hydrolysis of the esters for enzyme activity assays

Features and Benefits

  • Non-ionic surfactant
  • Improves solubility and dispersibility of substances
  • Excellent wetting properties
  • Enhances emulsification
  • High purity product for research applications

Preparation Note

Hydrogenated to reduce UV absorbance.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

comparable product

Product No.
Description
Pricing

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H319,H335,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

closed cup

Flash Point(C)

closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H A Van Lith et al.
European journal of biochemistry, 206(2), 527-535 (1992-06-01)
1. Substrate hydrolysis by two purified rabbit liver esterase-1 allozymes (ES-1A and ES-1B) was compared under conditions differing in substrate, pH and temperature. ES-1A and ES-1B activities had a similar pH and temperature dependency and similar thermal stability profile. 2.
Jose V Montoya G et al.
Experimental neurology, 220(2), 303-315 (2009-09-15)
Embryonic spinal cord motor neurons (MNs) can be maintained in vitro for weeks with a cocktail of trophic factors and muscle-derived factors under serum-containing conditions. Here we investigated the beneficial effects of muscle-derived factors in the form of muscle-conditioned medium
Luke H Chamberlain
FEBS letters, 559(1-3), 1-5 (2004-02-28)
The relative insolubility of lipid rafts in cold non-ionic detergents is the most widely used method to purify these fascinating membrane domains from intact cells or membranes. Most of what we know about lipid raft function has been derived from
Mammalian long-chain acyl-CoA synthetases.
Soupene E, Kuypers FA.
Exp. Biol. Med, 233, 507-521 (2008)
Ki-Bum Kim et al.
Methods in molecular biology (Clifton, N.J.), 424, 413-422 (2008-03-29)
Because lipid rafts are plasma membrane platforms mediating various cellular events such as in signal transduction, immunological response, pathogen invasion, and neurodegenerative diseases, protein identification in the rafts could provide important information to study their function. Here, we present an
View All Related Papers

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