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WTA2

Sigma-Aldrich

Complete Whole Transcriptome Amplification Kit

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Synonym(s):

transcriptome amplification kit

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55
Pricing and availability is not currently available.

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

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detection method

colorimetric

detection method

fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

endocrinological disorders, diabetes

relevant disease(s)

cancer

General description

WTA2 is optimized to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Whole Transcriptome Amplification (WTA) technology, allows for representative amplification of low nanogram quantities of total RNA in less than 4 hours without 3′-bias. Amplification products are suitable for applications such as qPCR, micro array analysis, and cloning. The WTA2 kit contains the polymerase needed to amplify the cDNA library.

Application

Complete Whole Transcriptome Amplification Kit is used for the following applications:
  • To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.[1]
  • Reverse transcription and cDNA amplification[2][3][4][5][6][7]
  • For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles[8]
  • Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)[9]
Suitable for use with downstream applications including:
  • qPCR
  • microarray analysis
  • cloning

Features and Benefits

  • Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required
  • Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling
  • Contains all needed components for cDNA amplification
  • Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias
  • Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism

Principle

The WTA2 process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. During this process, displaced single strands serve as new templates for primer annealing and extension. The resultant cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence. The 2nd step amplifies the cDNA library by PCR using WTA2 polymerase and a universal end primer to produce WTA2 product.

Kit Components Also Available Separately

Product No.
Description
SDS
  • Library Synthesis Enzyme
  • Library Synthesis Solution
  • Amplification Mix
  • Library Synthesis Buffer
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS
  • Amplification Enzyme
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology ReagentSDS

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Regulatory Information

常规特殊物品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCQ5847
    SLCP9475
    SLCQ8573
    SLCQ8820
    SLCP6950

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Bert Vanmechelen et al.
    Scientific reports, 8(1), 11171-11171 (2018-07-26)
    The family Arteriviridae harbors a rapidly expanding group of viruses known to infect a divergent group of mammals, including horses, pigs, possums, primates, and rodents. Hosts infected with arteriviruses present with a wide variety of (sub) clinical symptoms, depending on
    Genetic characterization of Tribevc virus and Kemerovo virus, two tick-transmitted human-pathogenic Orbiviruses
    Dilcher M, et al.
    Virology, 423, 68-76 (2012)
    Limited correlation of shotgun metagenomics following host depletion and routine diagnostics for viruses and bacteria in low concentrated surrogate and clinical samples
    Oechslin CP, et al.
    Frontiers in Cellular and Infection Microbiology, 2018, 375-375 null
    Aged stem cells reprogram their daily rhythmic functions to adapt to stress
    Solanas G, et al.
    Cell, 170, 678-692 (2017)
    137 differential gene expression of in vitro-matured bovine oocytes with or without a polar body.
    M. M. Pereira et al.
    Reproduction, Fertility, and Development, 24(1) , 181-181 (2011)
    View All Related Papers

    Articles

    WTA2 Amplicon Modification for NGS

    Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.

    Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

    The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

    Protocols

    Integration of Sigma® TransPlex® with Agilent Microarray

    TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

    Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

    WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

    Related Content

    Whole Transcriptome Amplification FAQs

    Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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