Skip to Content
Merck
CN

V6629

Vitamin B12

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%

Synonym(s):

α-(5,6-Dimethylbenzimidazolyl)cyanocobamide, CN-Cbl, Cyanocob(III)alamin, Cyanocobalamin

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C63H88CoN14O14P
CAS Number:
68-19-9
Molecular Weight:
1355.37
PubChem Substance ID:
24900763
eCl@ss:
34058004
UNSPSC Code:
12352205
NACRES:
NA.75
EC Number:
200-680-0
Beilstein/REAXYS Number:
4122889

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Vitamin B12, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%

InChI key

RMRCNWBMXRMIRW-QJRSUKKJSA-L

InChI

1S/C62H90N13O14P.CN.Co/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;1-2;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);;/q;;+2/p-2/t31-,34-,35-,36-,37+,41-,52-,53-,56-,57?,59-,60+,61+,62+;;/m1../s1

SMILES string

C/C(C1=N[C@]([C@]([C@H](CC(N)=O)[C@@]/2(C)CCC(NC[C@@H](OP([O-])(O[C@H]3[C@@H](O)[C@@H](N4C=[N]5C6=C4C=C(C)C(C)=C6)O[C@@H]3CO)=O)C)=O)([H])N([Co+]5C#N)C2=C(C)/C([C@@H](CCC(N)=O)C/7(C)C)=NC7=C/8)(C)[C@@](C)(CC(N)=O)[C@@H]1CCC(N)=O)=C9N=C8[C@@H](CCC(N)=O)[C@

biological source

synthetic ((organic))

product line

BioReagent

assay

≥98%

form

powder

mol wt

Mw 1355.37 g/mol

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

color

red to brown

pH

6

solubility

water: soluble 50 mg/mL, dark red

storage temp.

2-8°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Vitamin B12 has been used:
  • as a component of Erdschreiber′s medium
  • as a supplement of Dulbecco′s modified Eagle′s medium used for the isolation of ventricular myocytes from neonatal and adult rats
  • orally administered to male quails to study its role in reducing the negative effects of sodium selenite intoxication, in vivo
  • as a supplement in DMEM/ medium 199 (M199) medium for differentiation of human myoblasts.

Biochem/physiol Actions

Vitamin B12 is metabolized to its active form, methylcobalamin. Hindered vitamin B12 uptake is observed in pernicious anemia. Vitamin B12 is essential for erythroblast development. Pregnancy reduces the levels of vitamin B12. Aging reduces vitamin B12 absorption. It works as a coenzyme in fat, carbohydrate and protein metabolism. It is required for growth, genetic stability and survival of cells in vitro. Functions to support one-carbon metabolism. Present in many classical and serum-free formulations.

General description

Cyanocobalamin (B12) is a water soluble vitamin, commonly called as cobalamins. Dairy products, meat and eggs are the major sources of this vitamin.

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000523655
0000521040
0000521040
0000523655
0000520257

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Selenium toxicosis assessment (in vivo and in vitro) and the protective role of vitamin B12 in male quail (Coturnix coturnix)
Gad MA and El-Twab SMA
Environmental Toxicology and Pharmacology, 27(1), 7-16 (2009)
Vitamins, minerals and trace elements
Drugs During Pregnancy and Lactation (2015)
miRNA-204 drives cardiomyocyte proliferation via targeting Jarid2
Liang D, et al.
International Journal of Cardiology, 201, 38-48 (2015)
Effects of B Vitamins in Patients With Multiple Sclerosis
Nutrition and Lifestyle in Neurological Autoimmune Diseases, 261-265 (2017)
Vitamins for chronic disease prevention in adults: clinical applications
Fletcher RH and Fairfield KM
JAMA : The Journal of the American Medical Association, 287(23), 3127-3129 (2002)
View All Related Papers

Articles

Folic Acid and Tetrahydrofolates in Cell Culture

Explore folic acid importance in serum-free eukaryotic cell cultures, including CHO cells, and its role in media supplementation.

Vitamin B12, Cobalamin, in Cell Culture

Importance and uses of Vitamin B12 in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service