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V5764

Sigma-Aldrich

Anti-Vesicular GABA Transporter antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-VGAT

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About This Item

MDL number:
MFCD02264204
UNSPSC Code:
12352203
NACRES:
NA.77

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

potency

2-4 mg per mL effective concentration (determined by immunoblotting, using mouse brain and rat brain extracts)

mol wt

antigen ~55 kDa

species reactivity

rat, mouse

packaging

pkg of 200 μL

storage condition

avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)

concentration

~1.0 mg/mL

technique(s)

microarray: suitable
western blot: 2-4 μg/mL using mouse brain and rat brain extracts (S1 fraction)

UniProt accession no.

Q9H598
O35458
O35633

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SLC32A1(140679)
mouse ... Slc32a1(22348)
rat ... Slc32a1(83612)

General description

Vesicular GABA transporter (VGAT), also known as solute carrier family 32 member 1 (SLC32A1) or vesicular inhibitory amino acid transporter (VIAAT), is encoded by the gene localized on human chromosome 20q11.23. The encoded protein is highly expressed in both γ-aminobutyric acid -ergic (GABAergic) and glycinergic nerve endings.

Immunogen

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.

Application

Anti-Vesicular GABA Transporter antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blot
  • immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Vesicular GABA transporter (VGAT) plays a vital role in loading of presynaptic vesicles with glycine and γ-aminobutyric acid (GABA). VGAT is precisely involved in packing GABA into synaptic vesicles. In addition, it also has a crucial role in inhibitory synaptic transmission. Genetic variations in the gene leads to hyperekplexia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
039M4767V
21181012
093M4758V
025K4824

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Distribution and Posttranslational Modification of Synaptic ERα in the Adult Female Rat Hippocampus
Tabatadze N
Endocrinology, 154, 2013-2013 (2013)
Dopaminergic neurons inhibit striatal output via non-canonical release of GABA
Tritsch NX
Nature, 490, 262-266 (2012)
Forebrain Ischemia Triggers GABAergic System Degeneration in Substantia Nigra at Chronic Stages in Rats
Lin B
Cardiovascular Psychiatry and Neurology (2010)
Cyclin-Dependent Kinase 5 Is Involved in the Phosphorylation of Gephyrin and Clustering of GABAA Receptors at Inhibitory Synapses of Hippocampal Neurons
Kalbouneh H
PLoS ONE, 9 (2014)
B Lin et al.
Cardiovascular psychiatry and neurology, 2010, 506952-506952 (2010-10-29)
The long-term consequences of forebrain ischemia include delayed Parkinson's syndrome. This study revealed delayed neurodegeneration in the substantia nigra 8 weeks after 12.5 minutes of global ischemia in rat brain. Following neuronal loss of 30-40% in central and dorsolateral striatum
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