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V5387

Sigma-Aldrich

Anti-Vesicular Acetylcholine Transporter (VAChT) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD01634356
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

rat, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using 4% paraformaldehyde perfusion-fixed, floating tissue sections of rat brain
indirect immunofluorescence: 1:500 using NGF-treated PC12 cells
western blot: 1:2,000 using rat brain extract

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SLC18A3(6572)
rat ... Slc18a3(60422)

Specificity

The sequence is highly conserved in human VAChT.

Immunogen

synthetic peptide corresponding to the C-terminal of rat vesicular acetylcholine transporter (VAChT) (amino acids 512-530).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (2 papers)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
088K4804
077K4838
095K4751
049H4844
024K4774

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David Moszkowicz et al.
The journal of sexual medicine, 8(4), 1112-1122 (2011-01-29)
Little detailed information is available concerning autonomic and somatic nerve supply to the clitoris, potentially causing difficulties for nerve preservation during pelvic and perineal surgery. To identify the location and type (nitrergic, adrenergic, cholinergic and sensory) of nerve fibers in
Keith N Darrow et al.
The Journal of comparative neurology, 498(3), 403-414 (2006-07-28)
Immunostaining mouse cochleas for tyrosine hydroxylase (TH) and dopamine beta-hydroxylase suggests that there is a rich adrenergic innervation throughout the auditory nerve trunk and a small dopaminergic innervation of the sensory cell areas. Surgical cuts in the brainstem confirm these
N N Kumar et al.
Neuroscience, 170(3), 789-799 (2010-08-03)
Chemical coding of sympathetic preganglionic neurons (SPN) suggests that the chemical content of subpopulations of SPN can define their function. Since neuropeptides, once synthesized are transported to the axon terminal, most demonstrated chemical coding has been identified using immunoreactive terminals
Helene Bensoussan et al.
Toxicology, 261(1-2), 59-67 (2009-05-05)
Uranium is a heavy metal naturally present in the environment that may be chronically ingested by the population. Previous studies have shown that uranium is present in the brain and alters behaviour, notably locomotor activity, sensorimotor ability, sleep/wake cycle and
T L Powley et al.
Neuroscience, 186, 188-200 (2011-05-03)
Intramuscular arrays (IMAs), vagal mechanoreceptors that innervate gastrointestinal smooth muscle, have not been completely described structurally or functionally. To delineate more fully the architecture of IMAs and to consider the structure-function implications of the observations, the present experiment examined the
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