V4889
Anti-phospho-Vinculin (pTyr822) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human, chicken, rat, mouse
technique(s)
western blot: 1:1,000 using chick embryo fibroblasts (CEFs) transfected with activated Src.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr822)
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
General description
Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.
Immunogen
synthetic phosphopeptide derived from the region of vinculin that contains tyrosine 822.
Application
Anti-phospho-Vinculin (pTyr822) antibody produced in rabbit has been used in immunofluorescence staining.
Biochem/physiol Actions
Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading.In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Sex-specific association of rs4746172 of VCL gene with hypertension in two Han populations from Southern China.
Yu Q
Scientific Reports (2015)
Molecular mechanism of transglutaminase-2 in corneal epithelial migration and adhesion
Tong L
Biochimica et Biophysica Acta, 1833, 1304-1315 (2013)
Shun Shibata et al.
Cell reports, 25(6), 1668-1679 (2018-11-08)
The extracellular matrix plays a key role in stem cell maintenance, expansion, and differentiation. Laminin, a basement membrane protein, is a widely used substrate for cell culture including the growth of human induced pluripotent stem cells (hiPSCs). Here, we show
Tina Izard et al.
The Journal of biological chemistry, 291(6), 2548-2555 (2016-01-06)
The cytoskeletal protein vinculin is a major regulator of cell adhesion and attaches to the cell surface by binding to specific phospholipids. Structural, biochemical, and biological studies provided much insight into how vinculin binds to membranes, what components it recognizes
Lindsay B Case et al.
Nature cell biology, 17(7), 880-892 (2015-06-09)
Focal adhesions (FAs) link the extracellular matrix to the actin cytoskeleton to mediate cell adhesion, migration, mechanosensing and signalling. FAs have conserved nanoscale protein organization, suggesting that the position of proteins within FAs regulates their activity and function. Vinculin binds
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