biological source
bovine eye (lens)
Quality Level
Assay
≥98% (SDS-GE)
form
lyophilized powder
mol wt
~57 kDa
UniProt accession no.
storage temp.
−20°C
Gene Information
bovine ... VIM(280955)
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Other Notes
A component of mesenchymal cell intermediate filaments.
Reconstitution
When reconstituted with distilled water at 1 mg vimentin per mL, the solution will contain 10 mM sodium phosphate, pH 7.5, 2 mM dithiothreitol, 1 mM EDTA, 6 M urea, and 10 mM methylammonium chloride.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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H Bloemendal et al.
FEBS letters, 180(2), 181-184 (1985-01-28)
A novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M
Youngjoo Kwon et al.
PloS one, 6(4), e18872-e18872 (2011-04-29)
Epithelial ovarian cancer is an aggressive and deadly disease and understanding its invasion mechanisms is critical for its treatment. We sought to study the penetration/invasion of ovarian tumor cells into extracellular matrices (ECMs) using a fibroblast-derived three-dimensional (3D) culture model
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