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UPS2

Sigma-Aldrich

Proteomics Dynamic Range Standard Set

Protein Mass Spectrometry Calibration Standard

Synonym(s):

Dynamic Range Standards, Proteomics Standard Set

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1 SET
CN¥11,569.45
5 X 1 SET
CN¥46,289.42

CN¥11,569.45

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1 SET
CN¥11,569.45
5 X 1 SET
CN¥46,289.42

About This Item

EC Number:
232-650-8
UNSPSC Code:
12161503
NACRES:
NA.24

CN¥11,569.45

Please contact Customer Service for Availability
Request a Bulk Order

biological source

human

form

ready-to-use solution

quality

Protein Mass Spectrometry Calibration Standard

concentration

10.6 μg/ampule protein

technique(s)

mass spectrometry (MS): suitable

shipped in

wet ice

storage temp.

−20°C

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1 of 4

This Item
UPS1EMS0004EMS0005
Proteomics Dynamic Range Standard Set Protein Mass Spectrometry Calibration Standard

UPS2

Proteomics Dynamic Range Standard Set

Universal Proteomics Standard Set Protein Mass Spectrometry Calibration Standard

UPS1

Universal Proteomics Standard Set

SOLu-Trypsin recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

EMS0004

SOLu-Trypsin

SOLu-Trypsin Dimethylated Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution

EMS0005

SOLu-Trypsin Dimethylated

biological source

human

biological source

-

biological source

-

biological source

-

concentration

10.6 μg/ampule protein

concentration

-

concentration

-

concentration

-

form

ready-to-use solution

form

ready-to-use solution

form

ready-to-use solution

form

ready-to-use solution

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.

Application

Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes.[2] It has also been used as a standard to spike HeLa cells for label-free quantification.[3]
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]

Kit Components Also Available Separately

Product No.
Description
SDS
  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg

Signal Word

Danger

Hazard Statements

H315,H318,H334,H335,H360F

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    SLCR5904
    SLCR5903
    0000260162
    SLCN1435
    SLCM8684

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    Christina Ludwig et al.
    Molecular & cellular proteomics : MCP, 11(3), M111-M111 (2011-11-22)
    For many research questions in modern molecular and systems biology, information about absolute protein quantities is imperative. This information includes, for example, kinetic modeling of processes, protein turnover determinations, stoichiometric investigations of protein complexes, or quantitative comparisons of different proteins
    PANDA: A comprehensive and flexible tool for quantitative proteomics data analysis
    Chang C, et al.
    Bioinformatics, 35, 898-900 (2018)
    Andrew F Jarnuczak et al.
    Journal of proteome research, 15(9), 2945-2959 (2016-07-28)
    Quantitative mass spectrometry-based proteomics of complex biological samples remains challenging in part due to the variability and charge competition arising during electrospray ionization (ESI) of peptides and the subsequent transfer and detection of ions. These issues preclude direct quantification from
    Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
    Carpy A, et al.
    Molecular and Cellular Proteomics, 13, 1925-1925 (2014)
    Rosemary Yu et al.
    Nature communications, 11(1), 1881-1881 (2020-04-22)
    Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and
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