U8501
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast
Type X, lyophilized powder, ≥40 units/mg protein
Synonym(s):
UTP:α-D-glucose-1-phosphate uridylyltransferase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-811-2
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
Biological source:
bakers yeast
biological source
bakers yeast
type
Type X
form
lyophilized powder
specific activity
≥40 units/mg protein
composition
Protein, 30-60% modified Warburg-Christian
foreign activity
UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%, inorganic pyrophosphatase ≤0.5%
storage temp.
−20°C
Quality Level
General description
Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.
Application
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
- in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
- to quantify Suc synthase (SUS) enzyme activity in rice
- to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes
Biochem/physiol Actions
Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.
Physical form
Lyophilized, sulfate-free powder containing citrate buffer salt
Other Notes
One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Laura Bonofiglio et al.
Microbial drug resistance (Larchmont, N.Y.), 17(1), 75-81 (2010-12-07)
Prevalence of serotype 6B penicillin (PEN)-nonsusceptible Streptococcus pneumoniae significantly increased from 15.8% (1993-1997) to 67.3% (1998-2002) (p<0.001) in Argentina. Serogroup 6 ranks fourth among different capsular types within invasive isolates from Argentinean patients <6 years of age. To evaluate whether
Kieran Smallbone et al.
Methods in enzymology, 500, 355-370 (2011-09-29)
In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast.
Zhe Ma et al.
Molecular biology reports, 38(4), 2751-2760 (2010-11-26)
UDP-Glucose Pyrophosphorylase (EC 2.7.7.9, UGPase) plays an important role in Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) cell envelope Hyaluronic acid (HA) biosynthesis and it is also recognized as a virulence determinant in several bacterial species. HA is valuable biopolymer used
Himangi R Jayakar et al.
BMC microbiology, 11, 179-179 (2011-08-09)
A number of studies have revealed that Francisella tularensis (FT) suppresses innate immune responses such as chemokine/cytokine production and neutrophil recruitment in the lungs following pulmonary infection via an unidentified mechanism. The ability of FT to evade early innate immune
D J Sutor et al.
Clinica chimica acta; international journal of clinical chemistry, 86(3), 329-332 (1978-06-15)
The concentration of pyrophosphate in urine has been determined using the enzyme uridine-5'-diphosphoglucose pyrophosphorylase. The technique is relatively quick and easy to perform. Reproducibility of results was good, and results from recovery experiments were excellent. The mean concentration of pyrophosphate
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