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U7128

Sigma-Aldrich

Uricase from Arthrobacter globiformis

lyophilized powder, 15-30 units/mg protein (biuret)

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Synonym(s):
Urate oxidase, Urate:O2 oxidoreductase
CAS Number:
9002-12-4
Enzyme Commission number:
1.7.3.3 (BRENDA, IUBMB)
EC Number:
232-655-5
MDL number:
MFCD00082126
UNSPSC Code:
12352204
PubChem Substance ID:
24900663
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

15-30 units/mg protein (biuret)

mol wt

tetramer ~32 kDa

storage temp.

−20°C

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.(2)

Application

Uricase from Arthrobacter globiformis has been used for coating the electrodes in - biosensor fabrication for the detection of uric acid in urine samples. It has also been used for analysing the effect of Zn, Cu, Mg, and Mn ions on uricase.

Biochem/physiol Actions

Arthrobacter globiformis is a major producer of uricase or urate oxidase in the poultry manure and litter. Uricase hydrolyzes uric acid and causes ammonia volatilization in poultry manure. In the majority of mammals, uricase converts uric acid to allantoin, a soluble excretion product. Humans lack uricase due to its loss during the evolution of hominids. This increases the risk of humans towards gout, kidney stone, hypertension and diabetes due to increased circulating levels of uric acid.Uricase is an important enzyme that plays a role in the purine metabolic pathway.
Molecular weight: 4 × ~32 KDa (tetramer)
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Zn and Cu were shown to inhibit the activity of uricase.

Unit Definition

One unit will convert 1.0 μmole of uric acid to allantoin per min at pH 9.0 at 25 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.
Kanyong P, et al.
Analytical biochemistry, 428(1), 39-43 (2012)
Review of Uricase and Other Key Metabolites Produced by Microorganisms through Submerged Fermentation INTRODUCTION
Sandeep VC, et al.
Polymer Journal, 22, 1500-1517 (2023)
Uric acid: a danger signal from the RNA world that may have a role in the epidemic of obesity, metabolic syndrome, and cardiorenal disease: evolutionary considerations.
Seminars in Nephrology, 31(5), 394-399 (2011)
A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties
Andrew C N, et al.
PLoS ONE, 11(12) (2016)
Kavita R Hegde et al.
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde, 222(3), 194-198 (2008-05-24)
Oxidative stress is considered to be a significant risk factor in the pathogenesis of many eye diseases associated with aging. The purpose of this study was to investigate the susceptibility of retinal tissue to reactive oxygen species (ROS)-induced damage, and
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