U6510
Ubiquitin-Specific Protease 5 human
recombinant, expressed in Sf9 cells, ≥51% (SDS-PAGE)
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UNSPSC Code:
12352204
NACRES:
NA.54
recombinant
expressed in Sf9 cells
Quality Level
form
buffered aqueous glycerol solution
mol wt
96 kDa
concentration
≥0.20 mg/mL protein (Bradford)
≥51% (SDS-PAGE)
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
General description
Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (isoT), amino acids 2-858 (end) with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
The tumor suppressor gene P53 was shown to be a target of ubiquitin-specific protease 5.
Physical form
Solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 80 ng/μl Flag peptide, 3 mM DTT, and 20% Glycerol at pH 8.0.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Francisca E Reyes-Turcu et al.
The Journal of biological chemistry, 283(28), 19581-19592 (2008-05-17)
The conjugation of polyubiquitin to target proteins acts as a signal that regulates target stability, localization, and function. Several ubiquitin binding domains have been described, and while much is known about ubiquitin binding to the isolated domains, little is known
Saurabh Dayal et al.
The Journal of biological chemistry, 284(8), 5030-5041 (2008-12-23)
Both p53 and its repressor Mdm2 are subject to ubiquitination and proteasomal degradation. We show that knockdown of the deubiquitinating enzyme USP5 (isopeptidase T) results in an increase in the level and transcriptional activity of p53. Suppression of USP5 stabilizes
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