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U6504

Sigma-Aldrich

Urea

suitable for electrophoresis

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Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
200-315-5
MDL number:
MFCD00008022
UNSPSC Code:
12352111
PubChem Substance ID:
24900435
NACRES:
NB.22

Assay

≥98%

Quality Level

200

form

powder or crystals

technique(s)

electrophoresis: suitable

impurities

≤0.005% Ammonia

mp

132-135 °C (lit.)

solubility

H2O: 100 mg/mL

density

1.335 g/mL at 25 °C (lit.)

cation traces

Fe: ≤5 ppm
Pb: ≤5 ppm

absorption

≤0.06 at 260 at 5 M
≤0.06 at 280 at 5 M

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759) , CA2(760)
rat ... Ppm1a(24666)

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Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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