U3254
Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat
clone DECMA-1, ascites fluid, buffered aqueous solution
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Synonym(s):
Anti-E-Cadherin, Anti-LCAM
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biological source
rat
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
DECMA-1, monoclonal
form
buffered aqueous solution
contains
15 mM sodium azide
species reactivity
bovine, human, canine, mouse
technique(s)
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:1,600 using cultured MDCK cells
microarray: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDH1(999)
mouse ... Cdh1(12550)
General description
Monoclonal Anti-Uvomorulin/E-Cadherin (rat IgG1 isotype) is derived from the DECMA-1 hybridoma, produced by the fusion of rat myeloma cells and splenocytes from an immunized Lou rat. Uvomorulin protein was initially identified in embryonal carcinoma and is identical to E-Cadherin, liver-cell adhesion molecules (L-CAM), Cell CAM 80/120, and Activity-regulated cytoskeleton-associated protein 1 (ARC-1), each of which have been characterized in different systems. Uvomorulin/E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.
Specificity
Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.
The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in immunoblotting or immunohistochemical assays.
Immunogen
mouse embryonal carcinoma cell line PCC4 Aza R1.
Application
Monoclonal Anti-Uvomorulin/E-Cadherin has been used in immunofluorescence, immunoblotting, immunoprecipitation, immunohistochemistry, macromolecule permeability assay and agglomeration of two embryoid body assay.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
WGK
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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