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U211250

Sigma-Aldrich

DMT-2′Fluoro-dU Phosphoramidite

configured for (ÄKTA® and OligoPilot®)

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Synonym(s):
DMT-2′-Fluoro-dU amidite
Empirical Formula (Hill Notation):
C39H46FN4O8P
CAS Number:
146954-75-8
Molecular Weight:
748.78
MDL number:
MFCD11113919
UNSPSC Code:
12352200
PubChem Substance ID:
329827617
NACRES:
NA.21

biological source

non-animal source (no BSE/TSE risk)

Quality Level

300

product line

Proligo Reagents

Assay

≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)

form

powder

technique(s)

oligo synthesis: suitable

impurities

≤0.3% water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (reversed phase HPLC)
≤3% residual Solvent content

color

white to off-white

λ

conforms (UV/VIS Identity)

suitability

conforms to structure for H-NMR
conforms to structure for LC-MS

compatibility

configured for (ÄKTA® and OligoPilot®)

nucleoside profile

base: deoxyuridine
base protecting group: none
2' protecting group: fluoro
5' protecting group: DMT
deprotection: fast/standard

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5

InChI

1S/C39H46FN4O8P/c1-26(2)44(27(3)4)53(50-24-10-22-41)52-36-33(51-37(35(36)40)43-23-21-34(45)42-38(43)46)25-49-39(28-11-8-7-9-12-28,29-13-17-31(47-5)18-14-29)30-15-19-32(48-6)20-16-30/h7-9,11-21,23,26-27,33,35-37H,10,24-25H2,1-6H3,(H,42,45,46)/t33-,35-,36-,37-,53?/m1/s1

InChI key

HQHQPAYRJJMYQX-DJTPZYMWSA-N

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General description

DMT-2′Fluoro-dU Phosphoramidite is a 2′Fluoro phosphoramidite used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.Its key features are-
  • Can be employed together with DNA or RNA phosphoramidites
  • Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
  • Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
  • Consistent lot-to-lot high purity and performance
  • Manufactured under a certified ISO 9001 quality system

Application

DMT-2′Fluoro-dU Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry.

Legal Information

OligoPilot is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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