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TOX6

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Sulforhodamine B based

Synonym(s):

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

form

liquid

Quality Level

200

usage

 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

565 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

room temp

General description

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

Application

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

Biochem/physiol Actions

Dye binds to cellular protein and is then solubilized in base.

Signal Word

Danger

Hazard Statements

H226,H314,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?

    If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed.  This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.

  6. Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?

    Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension.  Otherwise, you need to centrifuge, remove the media and then fix.  Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
Sofia Oliveira-Pinto et al.
Bioorganic chemistry, 100, 103942-103942 (2020-05-26)
A selection of new chromeno[2,3-b]pyridines was prepared from chromenylacrylonitriles and N-substituted piperazines, using a novel and efficient synthetic procedure. The compounds were tested for their anticancer activity using breast cancer cell lines MCF-7, Hs578t and MDA-MB-231 and the non-neoplastic cell
Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
Skehan, P., et al.
Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
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