Cas9 Recombinant protein from Streptococcus pyogenes

Recombinant Cas9 proteins (∼160 KD) are ready-to-use reagents for genome engineering experiments. When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

The kit comes in three pack sizes:

• TGEN-CP-50UG
• TGEN-CP-250UG
• TGEN-CP-500UG

Each kit consists of:

• one vial of Cas9 recombinant protein
• one vial containing 1 mL of 1x dilution buffer
• one vial containing 1mL of Nuclease free water with glycerol

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

Use our CRISPR Selection Tool to order gRNA at sigma.com/crisprs

Know more about Cas9 Recombinant proteins

Lyophilized Recombinant _Sp_Cas9 protein

It is recommended that the lyophilized Cas9 protein be resuspended in the nuclease-free water containing 50% glycerol, that is provided in the kit, to get the desired concentration.
The solution should be allowed to incubate at room temperature (20−23 °C) for 10 minutes. Centrifuge immediately before use.