TDF100A
Total Dietary Fiber Assay Kit
sufficient for ~100 assays
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About This Item
NACRES:
NA.84
UNSPSC Code:
12164500
Product Name
Total Dietary Fiber Assay Kit, sufficient for ~100 assays
usage
sufficient for ~100 assays
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Quality Level
Application
This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.
Biochem/physiol Actions
Uses a combination of enzymatic and gravimetric methods. This procedure is based on the method published in Official Methods of Analysis, 15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).
Other Notes
Reagents for the determination of total dietary fiber.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
target_organs
Lungs, Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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