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Safety Information

T9777

Sigma-Aldrich

Trypsin-chymotrypsin inhibitor

lyophilized powder

Synonym(s):

Bowman-Birk Inhibitor

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1 L
¥727.65

¥727.65

Available to ship on2025年4月27日Details

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1 L
¥727.65

About This Item

CAS Number:
37330-34-0
MDL number:
MFCD00132532
UNSPSC Code:
12352202
NACRES:
NA.77

¥727.65

Available to ship on2025年4月27日Details

Product Name

Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder

biological source

Glycine max (soybean)

Quality Level

200

form

lyophilized powder

composition

Protein, 70-90% biuret

storage temp.

2-8°C

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Perchloric acid 0.01 M HClO4 in water (0.01N), eluent for IC

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Perchloric acid puriss. p.a., ACS reagent, packed in coated, shock- and leak-protected glass bottle, ≥60% (T)

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Perchloric acid

Perchloric acid ACS reagent, 60%

311413

Perchloric acid

concentration

0.01 M HClO4 in water (0.01N)

concentration

-

concentration

≥60% (T)

concentration

60%

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ion chromatography: suitable

technique(s)

toxicology assay: suitable

technique(s)

-

technique(s)

-

General description

Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.
Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops.[1] Trypsin-chymotrypsin has anticarcinogenic properties.[2]

Application

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.
Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay.[3] It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.[4]

Unit Definition

One trypsin unit = ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

Physical form

Lyophilized powder containing phosphate buffer salts, pH 7.6

Analysis Note

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

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    Lot/Batch Number
    0000424767
    0000424767
    0000409430
    0000389148
    0000389150

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    Alfonso Clemente et al.
    Molecular nutrition & food research, 54(3), 396-405 (2009-11-04)
    Bowman-Birk inhibitors (BBI) from soybean and related proteins are naturally occurring protease inhibitors with potential health-promoting properties within the gastrointestinal tract. In this work, we have investigated the effects of soybean BBI proteins on HT29 colon adenocarcinoma cells, compared with
    Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS
    Ruppenthal S, et al.
    PLoS ONE, 13(1), e0191734-e0191734 (2018)
    The Bowman-Birk inhibitor from soybeans as an anticarcinogenic agent
    Kennedy AR
    American Journal of Clinical Nutrition, 68(6), 1406S-1412S (1998)
    Evidence for ancient origins of Bowman-Birk inhibitors from Selaginella moellendorffii
    James AM, et al.
    Plant Cell, 29(3), 461-473 (2017)
    Yu Liu et al.
    Viruses, 10(10) (2018-10-17)
    The Bowman‒Birk inhibitor (BBI), a protease inhibitor derived from soybeans, has been extensively studied in anti-tumor and anti-inflammation research. We recently reported that BBI has an anti-HIV-1 property in primary human macrophages. Because HSV-2 infection plays a role in facilitating
    View All Related Papers

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