T9525
Tris-Borate-EDTA buffer
working solution
Synonym(s):
TBE buffer
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Select a Size
About This Item
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sterility
0.2 μm filtered
Quality Level
form
working solution
technique(s)
electrophoresis: suitable
impurities
DNase, RNase, Protease, none detected
suitability
suitable for gel electrophoresis (after dilution to working concentration)
application(s)
diagnostic assay manufacturing
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
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Related Categories
1 of 4
This Item | PHR1533 | C8503 | C8667 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - | technique(s) - |
| assay ≥99% | assay - | assay ≥92.5% (GC) | assay ≥99% |
| biological source ovine wool | biological source - | biological source sheep wool | biological source sheep wool |
| solubility H2O: 0.002 mg/mL, ethanol: soluble, Tween: soluble, serum: soluble | solubility water: soluble 0.00003 g/L at 20 °C | solubility water: soluble 0.00003 g/L at 20 °C | solubility water: soluble 0.00003 g/L at 20 °C |
| form powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. -10 to -25°C | storage temp. room temp | storage temp. −20°C |
Application
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
Other Notes
Preparation Note
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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