T8573
Monoclonal Anti-Topoisomerase I antibody produced in mouse
clone Mab1, purified immunoglobulin, buffered aqueous solution
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Mab1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
human
technique(s)
microarray: suitable
western blot: 1-2 μg/mL using A231 human kidney cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TOP1(7150)
General description
Monoclonal Anti-Topoisomerase I (mouse IgG1 isotype) is derived from the Mab1 hybridoma produced by the fusion of SP2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with human purified topoisomerase I. The protein family of DNA topoisomerases is divided into two types (type I and II). The type I enzymes include eukaryotic and bacterial topoisomerase I and III. Human DNA Topoisomerase I belong to the subtype IB enzymes. The protein has four distinct domains. There are four nuclear localization signals in the N-terminal domain. A linker domain of 77 amino acids follows the core domain. The fourth domain, which is 53 amino acids long, contains the active site Tyr723. Topoisomerase I (TOP1) gene is located on human chromosome 20q12.
Immunogen
purified human topoisomerase I
Application
Monoclonal Anti-Topoisomerase I antibody produced in mouse has been used in western blot analysis.
Biochem/physiol Actions
DNA topoisomerases are enzymes that control the amount of supercoiling in DNA. Without topoisomerases, DNA cannot replicate normally. Type I enzymes of DNA topoisomerases introduce transient single strand breaks in DNA. Recently it was reported that DNA topoisomerase I is involved in modulation of RNA and is present in retroviral particles, like human immunodeficiency virus (HIV1). Furthermore, the enzyme enhances HIV-1 cDNA production in reverse transcription assays in vitro. Topoisomerase I (TOP1) serves as swivel at the time of replication, transcription and recombination, which helps in releasing over winding of duplex DNA.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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DNA topoisomerase II and its growing repertoire of biological functions
Nitiss JL
Nature Reviews. Cancer, 9(5), 327-327 (2009)
Mechanisms for defining supercoiling set point of DNA gyrase orthologs I. A nonconserved acidic C-terminal tail modulates Escherichia coli gyrase activity
Tretter EM and Berger JM
The Journal of Biological Chemistry, 287(22), 18636-18644 (2012)
Tanshinone IIA potentiates the efficacy of 5-FU in Colo205 colon cancer cells in vivo through downregulation of P-gp and LC3-II.
Su C C
Experimental and Therapeutic Medicine, 3(3), 555-559 (2012)
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