T8387
Thiopropyl–Sepharose™ 6B
lyophilized powder
Synonym(s):
Thiopropyl−activated–Agarose
form
lyophilized powder
extent of labeling
18-31 μmol per mL
matrix active group
hydroxypropyl 2-pyridyl disulfide
swelling
1 g swells to 4-5 mL gel
storage temp.
2-8°C
Application
Thiopropyl Sepharose™ 6B is used in protein chromatography, affinity chromatography and hydrophobic interactions. Thiopropyl Sepharose™ 6B has been used for the removal of inhibitors from PCR extracts from evidence collected for law enforcement murder investigations. Thiopropyl Sepharose™ 6B has also been used to describe a method for the investigation of functional properties of distinct domains of viral thiol proteins, including the influenza virus membrane M1 protein.
Physical form
Lyophilized powder stabilized with lactose and dextran
Legal Information
Sepharose is a trademark of Cytiva
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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M Ehrat et al.
Clinical chemistry, 32(9), 1622-1630 (1986-09-01)
The S-peptide and S-protein fragments of ribonuclease S (RNase S, no EC no. assigned) have been immobilized onto separate Sepharose gels via a "leash" of polycytidylic acid substrate. Each of these gels releases its RNase fragment when treated with the
B C Hill et al.
The Journal of biological chemistry, 261(33), 15356-15359 (1986-11-25)
Subunit III was removed from beef heart cytochrome oxidase by incubation of the isolated enzyme at 25 degrees C for 24 h in lauryl maltoside buffer at a detergent to protein ratio of 10:1 (w:w). During the course of the
M Persson et al.
FEBS letters, 270(1-2), 41-44 (1990-09-17)
The gene encoding glucose dehydrogenase (EC 1.1.1.47) from Bacillus subtilis was inserted in a plasmid 1.0 kb downstream from a lac promoter, resulting in a 70-fold higher production of the enzyme when expressed in Escherichia coli. A glucose dehydrogenase mutant
G G Shutler et al.
Journal of forensic sciences, 44(3), 623-626 (1999-07-17)
The analysis of biological trace evidence from a reopened investigation into a 1991 murder from Vernon, B.C. revealed mixed human and dog bloodstains on blue jean pants that contained a PCR inhibitory substance. The presence of the inhibitory substance was
Erik Sørensen et al.
International journal of legal medicine, 117(4), 245-247 (2003-06-24)
PCR amplification of DNA from forensic samples often proves difficult due to the presence of inhibitors of the polymerase chain reaction. One possible way to remove PCR inhibitors from a DNA extract is the use of the affinity resin thiopropyl
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