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T8159
Tryptose Phosphate Broth solution
sterile-filtered, suitable for cell culture
Synonym(s):
TPB solution
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500 G
CN¥922.59
1 KG
CN¥1,016.24
2.5 KG
CN¥1,757.85
6 X 500 G
CN¥2,787.10
5 KG
CN¥3,039.60
12 KG
CN¥4,266.45
6 X 1 KG
CN¥4,733.38
4 X 2.5 KG
CN¥4,823.57
25 KG
CN¥8,527.66
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500 G
CN¥922.59
1 KG
CN¥1,016.24
2.5 KG
CN¥1,757.85
6 X 500 G
CN¥2,787.10
5 KG
CN¥3,039.60
12 KG
CN¥4,266.45
6 X 1 KG
CN¥4,733.38
4 X 2.5 KG
CN¥4,823.57
25 KG
CN¥8,527.66
About This Item
UNSPSC Code:
12352205
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
form
solution
concentration
29.5 g/L in deionized water
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
room temp
1 of 4
This Item | 798592 | 238597 | SX0760E |
|---|---|---|---|
| assay ≥99% | assay ≥99% | assay ≥99.0% | assay ≥99.0% |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| grade ACS reagent, anhydrous | grade ACS reagent, anhydrous | grade ACS reagent, anhydrous | grade - |
| mp 884 °C (lit.) | mp 884 °C (lit.) | mp 884 °C (lit.) | mp 884 °C (lit.) |
| pH 5.2-9.2 (25 °C, 5%) | pH 5.2-9.2 (25 °C, 5%) | pH 5.2-9.2 (25 °C, 5% in water) | pH 5.2-8.0 (20 °C, 50 g/L in H2O) |
Application
In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Components
Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
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Héctor Puente et al.
Frontiers in microbiology, 11, 1911-1911 (2020-09-26)
Coronaviruses (CoVs) cause severe respiratory, enteric, and systemic infections in a wide range of hosts, including humans and animals. Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, is the etiological agent of porcine epidemic diarrhea (PED), a
Dong-Hwan Kim et al.
Molecular biotechnology, 63(2), 140-149 (2021-01-03)
Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency
S N Saha et al.
Vaccine, 7(4), 357-363 (1989-08-01)
Studies were undertaken to develop a cheaper medium with indigenous sources of peptone and casein hydrolysate for continuous culture of BHK-21 (suspension) cells and production of foot-and-mouth disease (FMD) vaccine. Eleven batches of experimental media were prepared using different indigenous
S M Deutschmann et al.
Enzyme and microbial technology, 16(6), 506-512 (1994-06-01)
Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied
A I Josemans et al.
Annals of the New York Academy of Sciences, 969, 141-146 (2002-10-17)
The in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, was first achieved in 1985. Culture media were usually supplemented with serum and tryptose phosphate broth, both undefined components, contributing to great variability. Recently, we
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