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T7818

Sigma-Aldrich

Trizma® Pre-set crystals

BioPerformance Certified, pH 7.5, average Mw 150.6

Synonym(s):

Tris crystals

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

200

form

crystalline powder

mol wt

average Mw 150.6

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, NICKase, none detected
 endotoxin, tested
 total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

pH

7.50 (25 °C, 0.1 M)(lit.)
7.5

useful pH range

7.0-9.0

solubility

water: 0.66 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

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Application

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Certain pH electrodes do not give accurate results with Tris buffers.

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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