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T7408

Sigma-Aldrich

Triethylammonium bicarbonate buffer

1.0 M, pH 8.5±0.1

Synonym(s):

Triethylammonium hydrogen carbonate buffer

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About This Item

CAS Number:
Beilstein:
3624751
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

form

liquid

Quality Level

storage condition

(Tightly closed)

concentration

1.0 M

color

colorless

pH

8.5±0.1

density

1.02 g/mL at 25 °C (lit.)

suitability

suitable for LC-MS
suitable for mass spectrometry

application(s)

diagnostic assay manufacturing
microbiology
sample preparation

storage temp.

2-8°C

SMILES string

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI key

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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Application

Triethylammonium bicarbonate buffer is used in ion-exchange chromatography and electrophoresis.
It has also been used:
  • in trypsin digestion
  • as a component of lysis buffer
  • to adjust the pH of buffer for protein quantification

Buffer for use in ion-exchange chromatography and electrophoresis.

Biochem/physiol Actions

Triethylammonium bicarbonate buffer is useful in oligonucleotides purification for electrospray mass spectrometry analysis. It is also utilized in trypsin digestion buffers.

Other Notes

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comparable product

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Methods in Molecular Biology, 109-117 (2013)
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In proteomics, proteolytic peptides are often chemically modified to improve MS analysis, peptide identification, and/or to enable protein/peptide quantification. It is known that such chemical modifications can alter peptide fragmentation in collision induced dissociation MS/MS. Here, we investigated the fragmentation
Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
Hao P, et al.
Journal of Proteome Research, 14(2), 1308-1314 (2015)
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Hypothetical (HyP) and conserved HyP genes account for >30% of sequenced bacterial genomes. For the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough, 347 of the 3634 genes were annotated as conserved HyP (9.5%) along with 887 HyP genes (24.4%). Given the large

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