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T6791

Sigma-Aldrich

Trizma® base

BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
77-86-1
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
201-064-4
MDL number:
MFCD00004679
UNSPSC Code:
12352104
PubChem Substance ID:
24900405
NACRES:
NA.25

description

aminopeptidase substrate

Quality Level

400

product line

BioXtra

Assay

≥99.9% (titration)

form

crystalline

storage condition

dry at room temperature

impurities

≤0.005% Insoluble matter

ign. residue (900 °C)

≤0.01% (as SO4)

loss

≤0.5% loss on drying, 110°C

color

white

pH

10.5-12.0 (1 M in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

H2O: 1 M, colorless
water: 678 g/L at 20 °C

anion traces

chloride (Cl-): ≤0.005%
sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

SMILES string

NC(CO)(CO)CO

absorption

≤0.020 at 280 in H2O at 1 M
≤0.025 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing
life science and biopharma
sample preparation

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris base, also referred as THAM or Trizma® base, plays a vital role in various research applications as a biological buffer. It is commonly employed in buffer formulations like TAE and TBE buffers, thanks to its pKa of 8.06, which makes it suitable for maintaining pH within the physiological range (pH 7.0-9.0) for most living organisms. However, it is important to exercise caution when studying proteins, as Tris may interfere with the activity of certain enzymes. This versatile compound is indispensable in laboratories, where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Moreover, Tris base finds applications in biochemistry and molecular biology labs aiding in cell membrane permeability studies and buffer preparation.

Application

Tris buffer has been used to stabilize the pH, facilitating the measurement of cytotoxicity using the sulforhodamine B (SRB) colorimetric cytotoxicity assay in cancer research. Additionally, it was utilized in this study to regulate the pH of the incubation medium to assess the glucose secretion as a response mediated by glucocorticoids in metabolic pathways.

Features and Benefits

  • Suitable for Cell Membrane permeability studies and for buffer preparation in Biochemical and Molecular Biology Research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
  • Tested for the presence of Anionic and Cationic traces

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For additional information on our range of Biochemicals, please complete this form.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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