T6400
Tris-Borate-EDTA buffer
5× Concentrate
Synonym(s):
TBE buffer
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About This Item
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Quality Level
sterility
0.2 μm filtered
form
solution
impurities
DNase and RNase, none detected
pH
8.2-8.4 (25 °C)
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
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This Item | PPTG04700 | PPTH02500 | PPTH04700 |
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material plain filter, white filter, polypropylene membrane | material plain filter, polypropylene membrane, white filter | material plain filter, polypropylene membrane, white filter | material plain filter, polypropylene membrane, white filter |
| pore size 0.2 μm pore size, >40 % porosity | pore size 0.2 μm pore size, >40 % porosity | pore size 0.45 μm pore size, >40 % porosity | pore size 0.45 μm pore size, >40 % porosity |
| feature hydrophobic | feature hydrophobic | feature hydrophobic | feature hydrophobic |
| parameter >0.90 mL/min-cm2 air flow rate at 23 °C (typical), 1 h at 90 °C | parameter >0.90 mL/min-cm2 air flow rate (typical), 1 h at 90 °C | parameter >0.90 mL/min-cm2 air flow rate (typical), 1 h at 90 °C | parameter >0.90 mL/min-cm2 air flow rate (typical), 1 h at 90 °C |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
Application
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
Other Notes
Preparation Note
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Certificates of Analysis (COA)
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Protocols
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
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