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T5771

Sigma-Aldrich

Testosterone 3-(O-carboxymethyl)oxime: BSA - fluorescein isothiocyanate conjugate

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About This Item

MDL number:
MFCD00132415
UNSPSC Code:
51111800
NACRES:
NA.77

Assay

≥98.00% (TLC)

Quality Level

200

form

powder

drug control

regulated under CDSA - not available from Sigma-Aldrich Canada

extent of labeling

~3 mol FITC per mol BSA
~10 mol steroid per mol BSA

technique(s)

flow cytometry: suitable

solubility

water: 1.90-2.10 mg/mL, clear to faintly hazy, orange

shipped in

ambient

storage temp.

2-8°C

Application

Testosterone 3-(O-carboxymethyl)oxime: BSA-FITC has been used for flow cytometric assays in serum-free cell cultures2. It has also been used for fluorescence labeling experiments in L6 cells3.

Biochem/physiol Actions

Studies have reported that BSA-coupled testosterone can block the growth of LNCaP cells. Furthermore, it can activate Fas protein and cell death, as well as reduce the translocation, adhesion and incursion of iAR-negative, DU145 prostate cancer cells2.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H302 + H312 + H332,H351

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

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Rui Fu et al.
Journal of cellular physiology, 227(1), 98-107 (2011-03-05)
Androgens are known to modulate the skeletal muscle proliferation and differentiation processes. Recent in vitro studies have shown that dihydrotestosterone and anabolic steroids have functions in promoting the proliferation and differentiation of the mouse skeletal muscle myoblast C2C12 cell line
Anastassia Hatzoglou et al.
The Journal of clinical endocrinology and metabolism, 90(2), 893-903 (2004-12-09)
Nongenomic androgen actions imply mechanisms different from the classical intracellular androgen receptor (iAR) activation. We have recently reported the identification of a membrane androgen receptor (mAR) on LNCaP human prostate cancer cells, mediating testosterone signal transduction within minutes. In the
Yizhou Zhang et al.
Aging, 11(8), 2281-2294 (2019-04-22)
The non-genomic actions of androgen-induced synaptic plasticity have been extensively studied. However, the underlying mechanisms remain controversial. We recently found that testosterone-fetal bovine serum albumin (T-BSA), a cell membrane-impermeable complex, led to a rapid increase in the postsynaptic density 95
Synthesis of fluorescein labelled steroid hormone-albumin conjugates for the fluorescent histochemical detection of hormone receptors.
E Gaetjens et al.
Journal of steroid biochemistry, 13(8), 1001-1003 (1980-08-01)
Sha Li et al.
Molecular and cellular endocrinology, 414, 82-90 (2015-07-15)
Testosterone (T), the principal androgen, and its metabolite, dihydrotestosterone (DHT), are known to mediate their effects through binding to intracellular androgen receptors (iARs). In addition to their well-known genomic effects, androgens rapidly alter neuronal excitability through a non-genomic pathway mediated

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